ITR Laboratories Canada has established itself as a go-to facility for excellence in assessment of immunotoxicity. Not only do we offer a full range of standard assessments, we also have a team of PhD immunologists fully capable to provide the customized MOA endpoints specified in ICH S8.

CURRENT OFFERINGS INCLUDE:

1. TDAR

  • Validated in vivo models using T-dependent antigen (KLH) for both adult and juvenile animals
  • ELISA assay to assess the formation of specific antibodies

2. CELL MEDIATE IMMUNITY

  • Memory T cell response
  • Macrophage and neutrophil function assays
    • Phagocytosis
    • Oxidative burst
    • Migration
  • T and B cell mitogenesis

3. MOA SPECIFIC ENDPOINTS which are customized to the test item

4. ABSOLUTE LYMPHOCYTE COUNTS

  • Flow cytometric evaluation of lymphocyte lineages from various tissues; most commonly blood, spleen, lymph nodes or thymus.
  • Detailed analysis of cell phenotype
    • Lineage subsets
      • T/B/NK
      • T subsets (memory/naïve, Tregs)
      • Monocytes (classical/activated)
      • Plasmacytoid DCs
    • Activation markers
    • Intracellular protein expression and phosphorylation (ie. Ki67, FoxP3, pStat, cytokines)

5. NK CELL FUNCTIONAL ASSAY

  • Performed on blood or spleen